A genetically-encoded YFP sensor with enhanced chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane chloride movement by gerbil prestin (SLC26a5).

BackgroundChloride is the major anion in cells, with many diseases arising from disordered Cl- regulation.For the non-invasive investigation of Cl- flux, YFP-H148Q and its derivatives chameleon and Cl-Sensor previously were introduced as genetically encoded chloride indicators.Neither the Cl- sensitivity nor the pH-susceptibility of these modificat

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